In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 19, No. 6 ( 2023-6-23), p. e1011449-
Kurzfassung:
Malaria parasite release (egress) from host red blood cells involves parasite-mediated membrane poration and rupture, thought to involve membrane-lytic effector molecules such as perforin-like proteins and/or phospholipases. With the aim of identifying these effectors, we disrupted the expression of two Plasmodium falciparum perforin-like proteins simultaneously and showed that they have no essential roles during blood stage egress. Proteomic profiling of parasite proteins discharged into the parasitophorous vacuole (PV) just prior to egress detected the presence in the PV of a lecithin:cholesterol acyltransferase (LCAT; PF3D7_0629300). Conditional ablation of LCAT resulted in abnormal egress and a reduced replication rate. Lipidomic profiles of LCAT-null parasites showed drastic changes in several phosphatidylserine and acylphosphatidylglycerol species during egress. We thus show that, in addition to its previously demonstrated role in liver stage merozoite egress, LCAT is required to facilitate efficient egress in asexual blood stage malaria parasites.
Materialart:
Online-Ressource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1011449
DOI:
10.1371/journal.ppat.1011449.g001
DOI:
10.1371/journal.ppat.1011449.g002
DOI:
10.1371/journal.ppat.1011449.g003
DOI:
10.1371/journal.ppat.1011449.g004
DOI:
10.1371/journal.ppat.1011449.g005
DOI:
10.1371/journal.ppat.1011449.g006
DOI:
10.1371/journal.ppat.1011449.s001
DOI:
10.1371/journal.ppat.1011449.s002
DOI:
10.1371/journal.ppat.1011449.s003
DOI:
10.1371/journal.ppat.1011449.s004
DOI:
10.1371/journal.ppat.1011449.s005
DOI:
10.1371/journal.ppat.1011449.s006
DOI:
10.1371/journal.ppat.1011449.s007
DOI:
10.1371/journal.ppat.1011449.s008
DOI:
10.1371/journal.ppat.1011449.s009
DOI:
10.1371/journal.ppat.1011449.s010
DOI:
10.1371/journal.ppat.1011449.s011
DOI:
10.1371/journal.ppat.1011449.s012
DOI:
10.1371/journal.ppat.1011449.s013
DOI:
10.1371/journal.ppat.1011449.r001
DOI:
10.1371/journal.ppat.1011449.r002
DOI:
10.1371/journal.ppat.1011449.r003
DOI:
10.1371/journal.ppat.1011449.r004
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2023
ZDB Id:
2205412-1
