In:
cclm, Walter de Gruyter GmbH, Vol. 36, No. 12 ( 1998-12-05), p. 959-961
Abstract:
Denaturing gradient gel electrophoresis displays the highest detection rate among mutation scanning methods. In classical denaturing gradient gel electrophoresis the denaturant gradient range and migration times vary for every amplicon to be scanned, greatly affecting the routine application of the method. As an alternative, we developed double gradient denaturing gradient gel electrophoresis where a gradient of pore size is superimposed over the denaturing one, allowing maintenance of the zone-sharpening effect even over prolonged time runs, and adoption of identical run time conditions for all fragments analyzed. Here double gradient denaturing gradient gel electrophoresis has been applied to the analysis of a number of point mutations and polymorphisms located in several exons of three different genes, the cystic fibrosis transmembrane conductance regulator, the β-globin and the p53 genes.
Type of Medium:
Online Resource
ISSN:
1434-6621
DOI:
10.1515/CCLM.1998.165
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1998
detail.hit.zdb_id:
1492732-9