In:
Open Chemistry, Walter de Gruyter GmbH, Vol. 21, No. 1 ( 2023-04-19)
Abstract:
Plant extracts have always been used as an alternative source of antimicrobial compounds. The recent spread of multi-drug-resistant bacteria and their increased treatment costs necessitated the study of alternative, cheap sources. The family Euphorbiaceae has over 300 genera and is widely used in traditional medicine. Euphorbia triaculeata , E. fractiflexa , and E. inarticulata were selected to study the antibacterial activity of the methanolic extract against 13 Gram-positive Staphylococcus aureus strains (including methicillin-resistant S. aureus ) and 2 Gram-negative isolates, Escherichia coli and Klebsiella pneumoniae , by the Kirby Bauer Disc diffusion test. Paper discs with different concentrations of the extracts (100, 50, and 25 µg mL −1 ) were prepared, along with the methanol control and standard antibiotic control. A gas chromatography-mass spectrometry (GC/MS) analysis was done to study the phytochemical components present in the plant methanolic extracts. A total of 50 different phytochemical compounds with antibacterial activity were detected by GC/MS analysis of the plants. Twenty-five compounds were detected in E. inarticulata , 24 in E. triaculeata , and 21 in E. fractiflexa . Out of 37 compounds found in E. inarticulata and E. triaculeata , 12 (32.43%) were common to both. Eleven (22%) compounds were unique to E. inarticulata , while 9 (18%) compounds were unique to E. triaculeata , and 13 (26%) compounds were unique to E. fractiflexa . E. fractiflexa showed the best antibacterial activity against MRSA and Gram-negative bacteria. It also showed higher unique compounds with antibacterial activity (26%), followed by E. inarticulata (11, 22%). This is the first GC/MS analysis and antimicrobial activity report of E. triaculeata and E. fractiflexa .
Type of Medium:
Online Resource
ISSN:
2391-5420
DOI:
10.1515/chem-2022-0325
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
2023
detail.hit.zdb_id:
2825411-9
