In:
Journal of Perinatal Medicine, Walter de Gruyter GmbH, Vol. 45, No. 9 ( 2017-12-20)
Abstract:
We sought to determine the association between prenatal smoking status and expression of fetal brain regulatory genes. Methods: At delivery, we collected information from parturient women on prenatal smoking habits and analyzed salivary cotinine levels. We obtained neonatal umbilical cord blood and extracted total RNA. We then employed the quantitative polymerase chain reaction (QPCR) analyses and the comparative CT method to calculate the relative gene expression of selected fetal brain regulatory genes responsible for (1) brain growth (brain-derived neutrotrophic factor, BDNF), (2) myelination (proteolipidic protein 1, PLP1 and myelin basic protein, MBP), and (3) neuronal migration and cell-cell interactions during fetal brain development or RLN. The χ Results: Of the 39 maternal-infant dyads included in this study, 25.6% were non-smokers, 43.6% were passive smokers and 30.8% were active smokers. The results showed down-regulation of the selected fetal brain regulatory genes among active smokers. Conclusions: These findings represent preliminary evidence in humans that intrauterine tobacco exposure impacts fetal brain programming. Future studies are warranted to examine whether our findings represent potential mechanisms through which adverse childhood/adult-onset cognitive and behavioral outcomes that have been previously linked to intrauterine exposure occur.
Type of Medium:
Online Resource
ISSN:
1619-3997
,
0300-5577
DOI:
10.1515/jpm-2016-0279
Language:
Unknown
Publisher:
Walter de Gruyter GmbH
Publication Date:
2017
detail.hit.zdb_id:
1467968-1
