In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 34, No. 1-2 ( 1979-2-1), p. 76-86
Abstract:
A substance has been purified from isolated nuclei of Physarum polycephalum by equilibrium and velocity gradient centrifugations, ion exchange chromatography and gel filtration which has a high molecular weight, can be labeled in vivo with 32 P, is heat stable and resistant to amylases, proteases, nucleases and phosphodiesterase but is sensitive to phosphatases or hydrolysis. This material consists of phospate and glycerol. It selectively inhibits in vitro transcription of RNA polymerases, predominantly the homologous enzyme A by binding to the enzyme. In the presence of this inhibitor of transcription a stable RNA polymerase-template complex cannot be formed. Binding to and inactivation of RNA polymerase is reversible at high ionic strength.
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.1515/znc-1979-1-216
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1979
detail.hit.zdb_id:
2078107-6
SSG:
12
