In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 37, No. 10 ( 1982-10-1), p. 921-926
Abstract:
Free living cells of Rhizobium japonicum 61-A-101 and bacteroids from Glycine max var. Mandarin infected with the same strain utilized malonate with a substrate saturation greater than 10 -2 mol/l. At low concentrations of malonate (10 -5 mol/1) the free living cells were significantly more active in utilizing malonate than bacteroids. In bacteroids two substrate saturation ranges were found, one between 3 x 10 -4 mol/l and 10 -3 mol/l, the other at more than 10 -2 mol/l. Utilization of malonate was not affected by 10 to 100 times larger concentrations of either arabinose or xylose. 10 -3 mol/l succinate inhibited the utilization of malonate (10 -4 mol/l) completely in bacteroids, and by 90% in free living cells. Succinate utilization (10 -4 mol/1) was reduced in those cells exposed to 100 times higher malonate concentration only by 20-30% . Utilization and incorporation of pyruvate and 2-oxoglutarate into bacteroids was also only slightly affected by 100 times larger malonate concentration. Citrate utilization by bacteroids however was reduced by more than 70%. The rate of endoxidation of malonate as sole carbon source ( 14 CO 2 production from [2- 14 C] malonic acid)was about 1.5 μmol · h -1 • mg protein -1 and about half the rate with Pseudomonas putida and 70% o f the rate with Pseudomonas fluorescens under the same conditions (pH 6.0, 28 °C).
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.1515/znc-1982-1012
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1982
detail.hit.zdb_id:
2078107-6
SSG:
12
