In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 50, No. 7-8 ( 1995-8-1), p. 505-510
Abstract:
Two mutants of the cyanobacterium Anabaena variabilis impaired in the utilization or formation of molecular hydrogen have been obtained by nitroso-guanidine mutagenesis. Cultures of both mutants did not show alterations in the growth characteristics or in the heterocysts frequency but evolved molecular hydrogen from nitrogenase with enhanced rates. Activity measurements in extracts showed that one mutant (PK84) did not perform Na 2 S 2 O 4- dependent H 2 -formation and was, therefore, unable to express an active bidirectional hydrogenase. Both mutants (PK84, PK 17R) were characterized by lower activity of phenazine-methosulphate-dependent H 2 -uptake when extracts were assayed from younger cultures. In older cells, particularly when grown with nitrate in the medium , this H 2 -uptake activity was, how ever, enhanced. Both mutants are likely affected in regulatory hydrogenase genes. The mutant PK84 offers perspectives for potential applications in solar energy conversion programs.
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.1515/znc-1995-7-807
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1995
detail.hit.zdb_id:
2078107-6
SSG:
12
