In:
The EMBO Journal, Springer Science and Business Media LLC, Vol. 42, No. 8 ( 2023-04-17)
Abstract:
image Chromosome segregation relies on proper centromere‐kinetochore attachments to microtubules. This study shows that centromere/kinetochore dysfunction in yeast, caused by chromatinization with human histones, is rescued by DASH/Dam1c mutants that reduce the strength of kinetochore‐microtubule attachments. Histone humanization in yeast leads to centromere dysfunction, kinetochore declustering, and persistent aneuploidy. Alteration‐of‐function DASH/Dam1c mutants are adaptive to yeast with human chromatin. DASH/Dam1c mutants stabilize ploidy levels in histone‐humanized yeast. Ploidy stabilization is achieved by disrupting DASH/Dam1c oligomerization thereby weakening microtubule attachments.
Type of Medium:
Online Resource
ISSN:
0261-4189
,
1460-2075
DOI:
10.15252/embj.2022112600
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2023
detail.hit.zdb_id:
1467419-1
detail.hit.zdb_id:
586044-1
SSG:
12