In:
Molecular Systems Biology, EMBO, Vol. 19, No. 4 ( 2023-04-12)
Abstract:
image A novel approach is proposed to study the functional relationships between two critical mechanisms in cell signaling: PTMs and complex formation. This proteomic workflow is applied to investigate YAP1, a major signal integrator and effector of the Hippo pathway. Modules of YAP1 phospho‐proteoforms and their interactors were identified via AP‐BNPAGE. A causal relationship between site phosphorylation and complex formation was established. The control of YAP1 phosphorylation and complex formation was studied in cells lacking known pathway effectors. A model was developed to demonstrate how PTPN14 could inhibit YAP1 co‐transcriptional activity via augmenting ternary complex formation and phosphorylation by LATS1/2.
Type of Medium:
Online Resource
ISSN:
1744-4292
,
1744-4292
DOI:
10.15252/msb.202211024
Language:
English
Publisher:
EMBO
Publication Date:
2023
detail.hit.zdb_id:
2193510-5
SSG:
12
