In:
Acta Endocrinologica, Oxford University Press (OUP), Vol. 100, No. 1 ( 1982-05), p. 98-104
Kurzfassung:
Highly specific antibodies to 13, 14-dihydro-15-ketoprostaglandin F 2α (PGFM) were raised in rabbits. The animals were immunized with PGFM-bovine serum albumin (BSA)-conjugates. Prior to the incubation procedure PGFM was extracted by a rapid method with dichloromethane followed by column chromatography. The antisera dilution was 1:10000 and the cross-reactivity towards prostaglandin A 2 , E 2 , F 2α , 13, 14-dihydro-15-ketoprostaglandin E 2 and the 15-ketoprostaglandin E 2 and F 2α was 〈 1%. The limit of detection was 1.9 ± 0.6 pg/ml plasma over the standard range 1.9–250 pg. The intra- and inter-assay variations were 3.9 and 15%, respectively. PGFM was measured throughout the menstrual cycle in female volunteers. In normal ovulatory women (n = 3) plasma levels of PGFM varied between 65.6 to 107.1 pg/ml. No significant variations of plasma PGFM were seen during the cycle. In anovulatory women (n = 4) no difference of PGFM was found during the cycle. PGFM levels in hyperprolactinaemic but ovulating women tend to be higher than in anovulatory, and normoprolactinaemic subjects. These data strongly indicate that PGFM is not correlated with other hormonal parameters tested here in the normal and anovulatory cycles.
Materialart:
Online-Ressource
ISSN:
0804-4643
,
1479-683X
DOI:
10.1530/acta.0.1000098
Sprache:
Unbekannt
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
1982
ZDB Id:
1485160-X
