In:
Genetics, Oxford University Press (OUP), Vol. 166, No. 1 ( 2004-01-01), p. 237-242
Kurzfassung:
Recent advances in experimental analyses of the evolution of RNA secondary structures suggest a more complex scenario than that typically considered by Kimura's classical model of compensatory evolution. In this study, we examine one such case in more detail. Previous experimental analysis of long-range compensatory interactions between the two ends of Drosophila Adh mRNA failed to fit the classical model of compensatory evolution. To further investigate and verify long-range pairing in Drosophila Adh with respect to models of compensatory evolution and its potential functional role, we introduced site-directed mutations in the Drosophila melanogaster Adh gene. We explore two alternative hypotheses for why previous analysis of long-range compensatory interactions failed to fit the classical model. Specifically, we investigate whether the disruption of a conserved short-range pairing within Adh exon 2 has an effect on Adh expression or if there is a dual functional role of a conserved sequence in the 3′-UTR in both long-range pairing and the negative regulation of Adh expression. We find that a classical result was not observed due to the pleiotropic effect of changing a nucleotide involved in both long-range base pairing and the negative regulation of gene expression.
Materialart:
Online-Ressource
ISSN:
1943-2631
DOI:
10.1534/genetics.166.1.237
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
2004
ZDB Id:
1477228-0
SSG:
12
