In:
Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 8, No. 3 ( 2018-12-01), p. 901-904
Abstract:
Zinc is an essential trace element, which plays important roles in different biological activities. The aim of this study was to evaluate the effect of zinc on rabbit spermatozoa during in vitro conditions. Fresh semen was obtained from 8 sexually mature New Zealand White rabbits, which were bred at National Agricultural and Food Centre in Lužianky. Experimental groups were prepared by diluting the semen with two forms of zinc (ZnCl2, ZnSO4 .7 H2O) in five different concentration: ZA, ZA1 (4.883 mg Zn.l-1), ZB, ZB1 (9.766 mg Zn.l-1), ZC, ZC1 (19.531 mg Zn.l-1), ZD, ZD1 (39.063 mg Zn.l-1), ZE, ZE1 (78.125 mg Zn.l-1). Experimental samples were compared against control groups (ZK, ZK1). Semen was evaluated using the Computer Assisted Semen Analysis (CASA) at time intervals 0, 60, 120 and 180 minutes after incubation at 39°C. MTT test was used for determination of cell viability. Results of monitored motility parameters (motility, progressive motility and velocity curved line) showed decreasing trend in experimental samples. Significantly lower values of motility were observed in sample ZE, where percentage of motility decreased to 9.18±1.34% and after 180 minutes of incubation. Evaluation of spermatozoa velocity curved line detected significant (P
Type of Medium:
Online Resource
ISSN:
1338-5178
DOI:
10.15414/jmbfs.2018-19.8.3.901-904
Language:
Unknown
Publisher:
Slovak University of Agriculture in Nitra
Publication Date:
2018
detail.hit.zdb_id:
2641525-2
