In:
Stem Cells, Oxford University Press (OUP), Vol. 27, No. 1 ( 2009-01-01), p. 259-265
Kurzfassung:
Adipose tissue-derived stem cells (ASCs) exhibit immunosuppressive effects in allogeneic transplantation. However, there is no report that evaluates the in vivo immune-modulating effect of ASCs in an experimental allergic rhinitis (AR) model. We investigated whether ASCs migrate to the nasal mucosa in an AR mouse model and evaluated the immune-modulating effect of ASCs in the AR mouse model. Cultured ASCs (2 × 106) were injected i.v. before the first allergen challenge in the AR mouse model. Migration of ASCs to the nasal mucosa was evaluated by immunofluorescence. The immunomodulatory effects of ASCs were evaluated by nasal symptoms, histology, serum ovalbumin (OVA)-specific antibody, and the cytokine profile of the spleen. ASCs migrated to the nasal mucosa in the AR mouse model. ASCs significantly reduced allergic symptoms and inhibited eosinophilic inflammation in the nasal mucosa. ASCs significantly decreased the serum allergen-specific IgE level and the IgG1/IgG2a ratio and significantly increased the IgG2a level in the AR mouse model. ASCs inhibited interleukin (IL)-4 and IL-5 production from OVA-incubated splenocytes, but enhanced interferon-γ production. In conclusion, ASCs can migrate to the nasal mucosa in the AR mouse model and inhibit eosinophilic inflammation partly via shifting to a T-helper 1 (Th1) from a Th2 immune response to allergens.
Materialart:
Online-Ressource
ISSN:
1066-5099
,
1549-4918
DOI:
10.1634/stemcells.2008-0283
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
2009
ZDB Id:
2030643-X
ZDB Id:
1143556-2
ZDB Id:
605570-9
SSG:
12
