In:
Medical academic journal, ECO-Vector LLC, Vol. 19, No. 4 ( 2020-04-03), p. 93-102
Abstract:
Objective. We aimed to analyze the alterations of concentration and activity of iron metabolism proteins in samples obtained from rats with hyperglycemia induced by streptozotocin (STZ). Materials and methods. Concentration and activity of ceruloplasmin (Cp) and transferrin (Tf), concentration of glucose, fructosamine, hemoglobin, ferritin (Ft), and iron were measured in blood samples obtained from rats after injection of STZ or saline (control). To develop in-house ELISA, highly purified preparations of Cp, Tf, and Ft, as well as specific antibodies against these proteins, were obtained. Results. The hyperglycemia in rats after STZ injection was confirmed by increase in glucose and fructosamine serum concentrations. Increase in Cp concentration and decrease in specific ferroxidase activity of Cp, concentration of Tf and Ft were observed in hyperglycemic rats. However, the absence of changes in iron concentration and total iron binding capacity of serum is indicative of compensatory response. Conclusion. STZ-induced hyperglycemia in rats was characterized by alteration of activity and concentration of iron metabolism proteins, however, this alteration was negated by homeostatic response. The alterations of Cp and Tf concentrations observed in this study are similar to those in acute phase of inflammation.
Type of Medium:
Online Resource
ISSN:
2687-1378
,
1608-4101
DOI:
10.17816/MAJ19088-19081
DOI:
10.17816/MAJ19088-19082
DOI:
10.17816/MAJ19088-19083
DOI:
10.17816/MAJ19088-19092
DOI:
10.17816/MAJ19088-19093
Language:
Unknown
Publisher:
ECO-Vector LLC
Publication Date:
2020