In:
Acta Biochimica Polonica, Polskie Towarzystwo Biochemiczne (Polish Biochemical Society), Vol. 57, No. 4 ( 2010-11-29)
Abstract:
To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by the light strand promoter minor RNA. Confocal microscopy demonstrated ND5 probe spreading (less for other probes) in mitochondrial reticulum tubules but upon RNase A treatment all probes contoured mtDNA nucleoid localization. DNase I spread the signal over mitochondrial tubules. Future applications are discussed.
Type of Medium:
Online Resource
ISSN:
1734-154X
,
0001-527X
DOI:
10.18388/abp.2010_2425
Language:
Unknown
Publisher:
Polskie Towarzystwo Biochemiczne (Polish Biochemical Society)
Publication Date:
2010
detail.hit.zdb_id:
2086022-5