In:
HortScience, American Society for Horticultural Science, Vol. 30, No. 4 ( 1995-07), p. 878C-878
Abstract:
We compared the transcriptional properties of mitochondrial DNA (mtDNA) from a pair of male-sterile (S cytoplasm)/maintainer (N cytoplasm) lines together with the nuclear-restored plants. The transcriptional patterns of the mitochondrial genes, cob and cox1 , were different between N and S cytoplasm. We cloned and sequenced the cob gene from both N and S onion. The Scob gene diverged from the Ncob gene 56 bp 5' to the initiation codon. This upstream region of the Scob gene was homologous to a part of ORF1708 of the tobacco chloroplast DNA. We designed primers to amplify the upstream regions of the cob by PCR. A 414-bp DNA fragment was amplified from the total DNA of `2935A' (S cytoplasm) and a 130-bp DNA fragment was amplified from that of `2935B' (N cytoplasm) using a pair of primers specific to S cytoplasm and nor cytoplasm, respectively.
Type of Medium:
Online Resource
ISSN:
0018-5345
,
2327-9834
DOI:
10.21273/HORTSCI.30.4.878C
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
1995
detail.hit.zdb_id:
2040198-X
