In:
HortScience, American Society for Horticultural Science, Vol. 31, No. 5 ( 1996-09), p. 855-857
Abstract:
To our knowledge, there has been no published technique to produce consistently high-quality slides of somatic chromosomes of roses ( Rosa sp.). Therefore, various pretreatments, fixatives, digestions, stains, and maceration and squashing methods were tested to identify a procedure to produce clear, well-spread chromosomes from shoot tips. The best results were obtained after pretreatment in a mixture of 0.1% colchicine and 0.001 m 8-hydroxyquinoline for 4 h, and fixation in 2 acetone: 1 acetic acid (v/v) with 2% (w/v) polyvinylpyrrolidone. The darkest-stained chromosomes were obtained with carbol-fuchsin staining of air-dried cell suspensions that had been spread in 3 ethanol: 1 acetic acid (v/v).
Type of Medium:
Online Resource
ISSN:
0018-5345
,
2327-9834
DOI:
10.21273/HORTSCI.31.5.855
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
1996
detail.hit.zdb_id:
2040198-X