In:
BioTechniques, Future Science Ltd, Vol. 70, No. 6 ( 2021-06), p. 327-335
Abstract:
Method summary An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in combination with a specific commercial kit used to perform the DNAse treatment and optimal RNA purification on column. The method ensures optimal yield, good purity and integrity for analysis of high and low molecular weight RNA molecules.
Type of Medium:
Online Resource
ISSN:
0736-6205
,
1940-9818
DOI:
10.2144/btn-2021-0001
Language:
English
Publisher:
Future Science Ltd
Publication Date:
2021
detail.hit.zdb_id:
1496354-1
SSG:
12