In:
BioTechniques, Future Science Ltd, Vol. 73, No. 1 ( 2022-06), p. 34-46
Abstract:
METHOD SUMMARY To allow for downstream applications involving fungi in addition to bacteria and archaea, five DNA extraction kits were compared with a previously established, standardized protocol for extracting DNA for microbial community analysis. Across 10 diverse sample types, one extraction kit was found to perform comparably to or better than the standardized protocol. This conclusion is based on per-sample comparisons of DNA yield, the number of quality-filtered sequences generated, the limit of detection of microbial cells, microbial community alpha-diversity, beta-diversity, taxonomic composition and the extent of well-to-well contamination.
Type of Medium:
Online Resource
ISSN:
0736-6205
,
1940-9818
DOI:
10.2144/btn-2022-0032
Language:
English
Publisher:
Future Science Ltd
Publication Date:
2022
detail.hit.zdb_id:
1496354-1
SSG:
12