In:
Combinatorial Chemistry & High Throughput Screening, Bentham Science Publishers Ltd., Vol. 26, No. 3 ( 2023-03), p. 498-506
Abstract:
Intestinal ischemia-reperfusion (I/R) injury occurs in several clinical situations
and after intestinal transplantation. This study aimed to examine the role of rhubarb peony decoction (RPD) in intestinal I/R injury. Methods: Different concentrations of RPD were set to treat IEC-6 and Caco-2 cells. Cell proliferation
and apoptosis were measured by CCK-8 and flow cytometry assays. High-throughput transcriptome sequencing was performed on IEC-6 cells treated with hypoxia-reoxygenation (HR) or
HR and RPD. Results: RPD treatment significantly promoted the proliferation of IEC-6 and Caco-2 cells and inhibited
apoptosis. Sequencing results identified 109 significantly up-regulated genes and 36 significantly down-regulated genes in the RPD group. In addition, the results of western blot suggested
that HR induced the expression of c-Fos, and the treatment of RPD prevented the HR-induced c- Fos expression. Importantly, knockdown of c-Fos rescued the HR-inhibited cell proliferation and
HR-induced apoptosis. Conclusions: In conclusion, RPD was beneficial in protecting the survival of intestinal epithelial
cells under HR stress. Furthermore, the increase in c-Fos expression after HR stress was closely related to the proliferation and apoptosis of intestinal epithelial cells.
Type of Medium:
Online Resource
ISSN:
1386-2073
DOI:
10.2174/1386207325666220512140827
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2023
SSG:
15,3
