In:
Future Microbiology, Future Medicine Ltd, Vol. 13, No. 11 ( 2018-09), p. 1225-1246
Abstract:
Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. Results: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). Conclusion: Our prevalence data from Pakistan show that – as in other parts of the world – carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.
Type of Medium:
Online Resource
ISSN:
1746-0913
,
1746-0921
DOI:
10.2217/fmb-2018-0082
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2018
SSG:
12
