In:
Nanomedicine, Future Medicine Ltd, Vol. 14, No. 6 ( 2019-03), p. 689-705
Kurzfassung:
Aim: To develop a monoclonal antibody against dehydroepiandrosterone (DHEA) and miniaturize it, generating a single-chain antibody variable fragment (scFv) against DHEA as an adrenocortical carcinoma (ACC) marker. Material & methods: DHEA conjugated to keyhole limpet hemocyanin was used as an immunogen to obtain anti-DHEA hybridomas. Variable fragments were cloned from hybridoma 5B7 total RNA, and used to detect DHEA in normal adrenal tissue and ACC cells. Results: IgM monoclonal antibody was highly specific, and the recombinant scFv preserved parental antibody characteristics, allowing tissue localization of DHEA. Conclusion: Undefined small lesions are challenges for clinicians and impact clinical adrenocortical tumor management. Generating an anti-DHEA scFv facilitates development of imaging tests for early diagnosis of pediatric ACC.
Materialart:
Online-Ressource
ISSN:
1743-5889
,
1748-6963
DOI:
10.2217/nnm-2018-0230
Sprache:
Englisch
Verlag:
Future Medicine Ltd
Publikationsdatum:
2019
SSG:
15,3
