In:
Arhiv za bioloske nauke, National Library of Serbia, Vol. 66, No. 1 ( 2014), p. 261-271
Abstract:
Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament
(hPDL-MSCs) and characterized by their morphology, clonogenic efficiency,
proliferation and differentiation capabilities. hPDL-MSCs, derived from
normal impacted third molars, possessed all of the properties of MSC,
including clonogenic ability, high proliferation rate and multi-lineage
(osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential.
Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive
for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1,
fibronectin, vimentin, ?-SMA), and negative for hematopoietic stem cell
markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as
primitive and highly multipotent cells, showed high expression of embryonic
markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof
that cells with mesenchymal properties can be obtained from periodontal
ligament tissue. Although these cells should be further investigated to
determine their clinical significance, hPDL-MSCs are believed to provide a
renewable and promising cell source for new therapeutic strategies in the
treatment of periodontal defects.
Type of Medium:
Online Resource
ISSN:
0354-4664
,
1821-4339
Language:
English
Publisher:
National Library of Serbia
Publication Date:
2014
detail.hit.zdb_id:
2380842-1
