In:
Arhiv za bioloske nauke, National Library of Serbia, Vol. 66, No. 4 ( 2014), p. 1485-1492
Kurzfassung:
This paper presents the results of analysis of the genetic variability of
seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR
markers. Genomic DNA was isolated from seed tissue of all seven populations.
The concentration of DNA samples was within the range of 1-4 mg/ml. Different
PCR protocols were used depending on the type of SSR markers. The total
number of fragments obtained by SSR analysis with 4 selected primers was 17
(only bands of strong and medium intensity were considered), of which 6
fragments were polymorphic (35.29%). In order to analyze the genetic
similarity of the analyzed populations, graphs of correspondence analysis and
UPGMA clusters were produced. By comparative analysis of the obtained
dendrograms, the dependence of population genetic differentiation and spatial
distance was observed, i.e. their isolation by natural barriers. The results
indicate that in further research of interpopulation variability it is
necessary, when graphically interpreting genetic distances, to use both
methods of statistical analysis (UPGMA analysis and correspondence analysis).
Materialart:
Online-Ressource
ISSN:
0354-4664
,
1821-4339
Sprache:
Englisch
Verlag:
National Library of Serbia
Publikationsdatum:
2014
ZDB Id:
2380842-1
