In:
Srpski arhiv za celokupno lekarstvo, National Library of Serbia, Vol. 141, No. 3-4 ( 2013), p. 178-186
Kurzfassung:
Introduction. Mesenchymal stem cells (MSCs) are a promising tool for
regenerative medicine, but due to the heterogeneity of their populations,
different sources and isolation techniques, the characteristics defining MSCs
are inconsistent. Objective. The aim of this study was to compare the
characteristics of MSCs derived from two different human tissues: peripheral
blood (PB-MSCs) and umbilical cord Wharton?s Jelly (UC-MSCs). Methods. The
PB-MSC and UC-MSC were isolated by adherence to plastic after
gradient-density separation or an explant culture method, respectively, and
compared regarding their morphology, clonogenic efficiency, proliferating
rates, immunophenotype and differentiation potential. Results. MSCs derived
from both sources exhibit similar morphology, proliferation capacity and
multilineage (osteogenic, chondrogenic, adipogenic and myogenic)
differentiation potential. Differences were observed in the clonogenic
capacity and the immunophenotype, since UC-MSCs showed higher CFU-F
(colony-forming units-fibroblastic) cloning efficiency, as well as higher
embryonic markers (Nanog, Sox2, SSEA4) expression. When additional surface
antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34,
CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and
?-smooth muscle actin), most appeared to have similar epitope profiles
irrespective of MSC source. Conclusion. The results obtained demonstrated
that both MSCs represent good alternative sources of adult MSCs that could be
used in cell therapy applications.
Materialart:
Online-Ressource
ISSN:
0370-8179
,
2406-0895
DOI:
10.2298/SARH1304178T
Sprache:
Englisch
Verlag:
National Library of Serbia
Publikationsdatum:
2013
ZDB Id:
2577665-4