In:
POINT OF VIEW. EAST – WEST, Mediprint, , No. 2 ( 2021-05-31), p. 68-71
Kurzfassung:
Purpose. To compare the transparency of dehydrated and non-dehydrated tissue-engineered corneas for cryopreservation. Materials and methods. Assessment transparency of tissue-engineered corneas using spectrophotometry. For comparison, three groups were performed: 1 - control group (fresh lenticules), 2 – NaCl– group (dehydrated tissue-engineered corneas), 3 – NaCl+ group (non-dehydrated tissue-engineered corneas). The spectrophotometer data was evaluated in 2 stages. At stage 1, the transparency of the control group was measured (n=30). At the second stage, the transparency of two experimental groups after storage in DMSO (groups NaCl- and NaCl+) was investigated. Results. In the NaCl+ group, the transparency slightly decreased compared to the control, and the protocol using NaCl– was the closed to the control. Conclusion. In general, after thawing the NaCl– and NaCl+ groups slightly decrease their transparency in comparison with the control, so both groups can replace each other. Key words: storage, cryopreservation, lenticule, cornea, decellularization, tissue engineering.
Materialart:
Online-Ressource
ISSN:
2410-1257
Originaltitel:
Поиск оптимальных условий для хранения роговичных тканеинженерных конструкций
DOI:
10.25276/2410-1257-2021-2
DOI:
10.25276/2410-1257-2021-2-68-71
Sprache:
Unbekannt
Verlag:
Mediprint
Publikationsdatum:
2021
