In:
Acta Veterinaria Brno, University of Veterinary Sciences Brno, Vol. 79, No. 3 ( 2010), p. 453-457
Abstract:
The aim of this study was to evaluate the amount of Endothelin-1 (ET-1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET-1 gene expression in pathogenesis of mechanically induced vasospasm. The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs. Laser Doppler was used for peripheral blood flow measurement. Specimens from the vasospastic vessel (group of specimens B) and from the flap border with no vasospasm (control group A) were taken 2 h after the stimulus initiation. Detection of ET-1 mRNA by Quantitative Real-Time RT-PCR was performed. β-actin was selected as an acceptable reference gene. Relative gene expression data were given as the n-fold change in transcription of target genes normalized to the endogenous control. Relative gene expressions and time indicators of vasospasm were compared in both groups. No significant difference of the ET-1 gene expressions was found between groups A and B ( p = 0.505). No correlation between the duration of vasospasm and ET-1 gene expression was found as well ( p = 0.299). In conclusion, the expression of the ET-1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased. In this study, the causality of the vasospasm pathogenesis and gene expression of ET-1 was not proven.
Type of Medium:
Online Resource
ISSN:
0001-7213
,
1801-7576
DOI:
10.2754/avb201079030453
Language:
English
Publisher:
University of Veterinary Sciences Brno
Publication Date:
2010
detail.hit.zdb_id:
2106644-9
SSG:
22
