In:
Eurosurveillance, European Centre for Disease Control and Prevention (ECDC), Vol. 25, No. 3 ( 2020-01-23)
Abstract:
Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence.
Type of Medium:
Online Resource
ISSN:
1560-7917
DOI:
10.2807/1560-7917.ES.2020.25.3.1900765
Language:
English
Publisher:
European Centre for Disease Control and Prevention (ECDC)
Publication Date:
2020
detail.hit.zdb_id:
2059112-3