In:
CLINICAL AND EXPERIMENTAL MORPHOLOGY, FSBSI Research Institute of Human Morphology, Vol. 9, No. 4 ( 2020), p. 12-23
Abstract:
Introduction. The pathogenesis of the novel coronavirus infection COVID-19 is being actively studied. Nevertheless, our up-to-date knowledge of lymphoid tissue response in the secondary immune organs dur-ing severe COVID-19 remains extremely limited. The aim of the study was to evaluate patterns of immu-nomorphological alterations in lymph nodes in patients with severe COVID-19 and to assess lymphocytes functional activity in them. Materials and methods. Lymph node tissue (autopsy material) from 17 deceased patients with severe COVID-19 was examined by histological and immunohistochemical methods using antibodies to CD4, CD8, CD20, CD30, CD123, CD138, PD-1. Results. Examined lymph nodes demonstrated lymphoid follicles reduction and paracortex expansion with reactive plasmacytosis and extrafollicular B-cell activation as well as sinus histiocytosis, variable hemophago-cytic cells, and blood vessel congestion. Red thrombi were observed in some lymph nodes. Hemorrhages in the stroma were frequent, and massive hemorrhages were found in individual nodes. Immunohistochemical study revealed CD4+ T-helpers predominance in the paracortex andcytotoxic CD8+ lymphocytes depletion together with an increase in the expression of both the PD-1 suppressor protein and the CD30 activation anti-gen on the lymphocyte surface. CD123-positive plasmacytoid dendritic cells resided in sinuses in abundance. Conclusion. Demonstrated B-associated zone reduction and cytotoxic T-lymphocytes depletion with an up-regulation of PD-1 expression in the lymph nodes in patients with severe COVID-19 indicate immune response exhaustion. At the same time, observed significant reactive plasmacytosis with the presence of numerous T-helper cells constitutes a morphological substrate of the humoral immunity. These findings might indicate the ineffec-tiveness of the humoral response at late stages of COVID-19 infection in context of cytotoxic immunity failure. Keywords: novel coronavirus infection, SARS-CoV-2, COVID-19, B- and T- immune response, lymph node
Type of Medium:
Online Resource
ISSN:
2226-5988
,
2686-6749
Uniform Title:
Патоморфологическая и иммуногистохимическая характеристика изменений лимфатических узлов у умерших от COVID-19 по данным аутопсий
DOI:
10.31088/CEM2020.9.4
DOI:
10.31088/CEM2020.9.4.12-23
Language:
Unknown
Publisher:
FSBSI Research Institute of Human Morphology
Publication Date:
2020
