In:
Uludağ Üniversitesi Tıp Fakültesi Dergisi, Uludag Universitesi Tip Fakultesi Dergisi, Vol. 48, No. 1 ( 2022-04-01), p. 77-81
Abstract:
Semen liquefaction is a proteolytic process in which semen is liquefied by the enzymatic activity of proteases derived from the prostate. Different practices are adopted among assisted reproduction laboratories for semen liquefaction. Effects of different temperatures to sperm viability due to osmotic changes are unknown. The aim of this methodological study is to reveal the changes in semen osmolality and sperm viability when liquefied at different temperatures. Male patients who applied to an Assisted Reproduction Technologies Center for semen analysis or intrauterine insemination were included to the study. Semen samples from 15 individuals were divided into two groups before liquefaction and were incubated at 37°C or room temperature. For each sample, osmolality measurement was performed with freezing point depression osmometer. Sperm motility was determined by the Makler counting chamber and motility types were classified as specified in the Guideline for Semen Analysis published by the World Health Organization. Sperm viability was determined by eosin-nigrosine staining following liquefaction. Student’s t-test was utilized to compare groups and the significance level was set at p
Type of Medium:
Online Resource
ISSN:
1300-414X
DOI:
10.32708/uutfd.1082243
Language:
Unknown
Publisher:
Uludag Universitesi Tip Fakultesi Dergisi
Publication Date:
2022
