In:
Frontiers in Endocrinology, Frontiers Media SA, Vol. 12 ( 2021-8-4)
Abstract:
In recent decades, the prevalence of obesity has been rising. One of the major characteristics of obesity is fat accumulation, including hyperplasia (increase in number) and hypertrophy (increase in size). After histological staining, it is critical to accurately measure the number and size of adipocytes for assessing the severity of obesity in a timely fashion. Manual measurement is accurate but time-consuming. Although commercially available adipocyte counting tools, including AdipoCount, Image-Pro Plus, and ImageJ were helpful, limitations still exist in accuracy and time consuming. In the present study, we introduced the protocol of combined usage of these tools and illustrated the process with histological staining slides from adipose tissues of lean and obese mice. We found that the adipocyte sizes quantified by the tool combination were comparable as manual measurement, whereas the combined methods were more efficient. Besides, the recognition effect of monochrome segmentation image is better than that of color segmentation image. Overall, we developed a combination method to measure adipocyte sizes accurately and efficiently, which may be helpful for experimental process in laboratory and also for clinic diagnosis.
Type of Medium:
Online Resource
ISSN:
1664-2392
DOI:
10.3389/fendo.2021.642000
DOI:
10.3389/fendo.2021.642000.s001
DOI:
10.3389/fendo.2021.642000.s002
DOI:
10.3389/fendo.2021.642000.s003
DOI:
10.3389/fendo.2021.642000.s004
DOI:
10.3389/fendo.2021.642000.s005
DOI:
10.3389/fendo.2021.642000.s006
DOI:
10.3389/fendo.2021.642000.s007
DOI:
10.3389/fendo.2021.642000.s008
DOI:
10.3389/fendo.2021.642000.s009
DOI:
10.3389/fendo.2021.642000.s010
DOI:
10.3389/fendo.2021.642000.s011
DOI:
10.3389/fendo.2021.642000.s012
DOI:
10.3389/fendo.2021.642000.s013
DOI:
10.3389/fendo.2021.642000.s014
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2592084-4