In:
Frontiers in Immunology, Frontiers Media SA, Vol. 15 ( 2024-3-19)
Abstract:
Deciphering cellular components and the spatial interaction network of the tumor immune microenvironment (TIME) of solid tumors is pivotal for understanding biologically relevant cross-talks and, ultimately, advancing therapies. Multiplexed tissue imaging provides a powerful tool to elucidate spatial complexity in a holistic manner. We established and cross-validated a comprehensive immunophenotyping panel comprising over 121 markers for multiplexed tissue imaging using MACSima™ imaging cyclic staining (MICS) alongside an end-to-end analysis workflow. Applying this panel and workflow to primary cancer tissues, we characterized tumor heterogeneity, investigated potential therapeutical targets, conducted in-depth profiling of cell types and states, sub-phenotyped T cells within the TIME, and scrutinized cellular neighborhoods of diverse T cell subsets. Our findings highlight the advantage of spatial profiling, revealing immunosuppressive molecular signatures of tumor-associated myeloid cells interacting with neighboring exhausted, PD1 high T cells in the TIME of hepatocellular carcinoma (HCC). This study establishes a robust framework for spatial exploration of TIMEs in solid tumors and underscores the potency of multiplexed tissue imaging and ultra-deep cell phenotyping in unraveling clinically relevant tumor components.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2024.1383932
DOI:
10.3389/fimmu.2024.1383932.s001
DOI:
10.3389/fimmu.2024.1383932.s002
DOI:
10.3389/fimmu.2024.1383932.s003
DOI:
10.3389/fimmu.2024.1383932.s004
DOI:
10.3389/fimmu.2024.1383932.s005
DOI:
10.3389/fimmu.2024.1383932.s006
DOI:
10.3389/fimmu.2024.1383932.s007
DOI:
10.3389/fimmu.2024.1383932.s008
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2024
detail.hit.zdb_id:
2606827-8