In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 14 ( 2023-4-18)
Kurzfassung:
Huanglongbing (HLB) is a global devastating citrus disease that is mainly caused by “ Candidatus Liberibacter asiaticus” ( C Las). It is mostly transmitted by the insect Asian citrus psyllid (ACP, Diaphorina citri ) in a persistent and proliferative manner. C Las traverses multiple barriers to complete an infection cycle and is likely involved in multiple interactions with D. citri . However, the protein–protein interactions between C Las and D. citri are largely unknown. Here, we report on a vitellogenin-like protein (Vg_VWD) in D. citri that interacts with a C Las flagellum (flaA) protein. We found that Vg_VWD was upregulated in C Las-infected D. citri. Silencing of Vg_VWD in D. citri via RNAi silencing significantly increased the C Las titer, suggesting that Vg_VWD plays an important role in the C Las –D. citri interaction. Agrobacterium -mediated transient expression assays indicated that Vg_VWD inhibits BAX- and INF1-triggered necrosis and suppresses the callose deposition induced by flaA in Nicotiana benthamiana . These findings provide new insights into the molecular interaction between C Las and D. citri .
Materialart:
Online-Ressource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2023.1119619
DOI:
10.3389/fmicb.2023.1119619.s001
DOI:
10.3389/fmicb.2023.1119619.s002
DOI:
10.3389/fmicb.2023.1119619.s003
DOI:
10.3389/fmicb.2023.1119619.s004
DOI:
10.3389/fmicb.2023.1119619.s005
Sprache:
Unbekannt
Verlag:
Frontiers Media SA
Publikationsdatum:
2023
ZDB Id:
2587354-4
