In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 14 ( 2023-5-5)
Abstract:
Eight Gram-negative, aerobic, motile with paired polar flagella and rod-shaped bacteria were isolated from six tobacco fields in Yunnan, PR China. 16S rRNA gene sequence analysis revealed that all the strains belonged to the genus Ralstonia . Among them, strain 22TCCZM03-6 had an identical 16S rRNA sequence to that of R. wenshanensis 56D2 T , and the other strains were closely related to R. pickettii DSM 6297 T (98.34–99.86%), R. wenshanensis 56D2 T (98.70–99.64%), and R. insidiosa CCUG 46789 T (97.34–98.56%). Genome sequencing yielded sizes ranging from 5.17 to 5.72 Mb, with overall G + C contents of 63.3–64.1%. Pairwise genome comparisons showed that strain 22TCCZM03-6 shared average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values above the species cut-off with R. wenshanensis 56D2 T , suggesting that strain 22TCCZM03-6 is a special strain of the R. wenshanensis . Five strains, including 21MJYT02-10 T , 21LDWP02-16, 22TCJT01-1, 22TCCZM01-4, and 22TCJT01-2, had ANI values & gt;95% and dDDH values & gt;70% when compared with each other. These five strains had ANI values of 73.32–94.17% and dDDH of 22.0–55.20% with the type strains of the genus Ralstonia individually, supporting these five strains as a novel species in the genus Ralstonia . In addition, strains 21YRMH01-3 T and 21MJYT02-11 T represent two independent species. They both had ANI and dDDH values below the thresholds for species delineation when compared with the type species of the genus Ralstonia . In strains 21YRMH01-3 T and 21MJYT02-10 T , the main fatty acids were summed features 3, 8, and C 16:0 ; however, strain 21MJYT02-11 T contained C 16:0 , cyclo-C 17:0 , and summed features 3 as major fatty acids. The main polar lipids, including diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine, were identified from strains 21YRMH01-3 T , 21MJYT02-10 T , and 21MJYT02-11 T . The ubiquinones Q-7 and Q-8 were also detected in these strains, with Q-8 being the predominant quinone. Based on the above data, we propose that the eight strains represent one known species and three novel species in the genus Ralstonia , for which the names Ralstonia chuxiongensis sp. nov., Ralstonia mojiangensis sp. nov., and Ralstonia soli sp. nov. are proposed. The type strains are 21YRMH01-3 T (=GDMCC 1.3534 T = JCM 35818 T ), 21MJYT02-10 T (=GDMCC 1.3531 T = JCM 35816 T ), and 21MJYT02-11 T (=GDMCC 1.3532 T = JCM 35817 T ), respectively.
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2023.1179087
DOI:
10.3389/fmicb.2023.1179087.s001
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2587354-4
