In:
Frontiers in Neuroanatomy, Frontiers Media SA, Vol. 16 ( 2022-12-22)
Abstract:
The duration of postsurgical pain is closely correlated with perioperative stress. Most patients suffer short-term sleep disorder/deprivation before and/or after surgery, which leads to extended postsurgical pain by an undetermined mechanism. The paraventricular thalamus (PVT) is a critical area that contributes to the regulation of feeding, awakening, and emotional states. However, whether the middle PVT is involved in postoperative pain or the extension of postoperative pain caused by perioperative sleep deprivation has not yet been investigated. Methods We established a model of postoperative pain by plantar incision with perioperative rapid eye movement sleep deprivation (REMSD) 6 h/day for 3 consecutive days in mice. The excitability of the CaMKIIα + neurons in the middle PVT (mPVT CaMKIIα ) was detected by immunofluorescence and fiber photometry. The activation/inhibition of mPVT CaMKIIα neurons was conducted by chemogenetics. Results REMSD prolonged the duration of postsurgical pain and increased the excitability of mPVT CaMKIIα neurons. In addition, mPVT CaMKIIα neurons showed increased excitability in response to nociceptive stimuli or painful conditions. However, REMSD did not delay postsurgical pain recovery following the ablation of CaMKIIα neurons in the mPVT. The activation of mPVT CaMKIIα neurons prolonged the duration of postsurgical pain and elicited anxiety-like behaviors. In contrast, inhibition of mPVT CaMKIIα neurons reduced the postsurgical pain after REMSD. Conclusion Our data revealed that the CaMKIIα neurons in the mPVT are involved in the extension of the postsurgical pain duration induced by REMSD, and represented a novel potential target to treat postoperative pain induced by REMSD.
Type of Medium:
Online Resource
ISSN:
1662-5129
DOI:
10.3389/fnana.2022.1074310
DOI:
10.3389/fnana.2022.1074310.s001
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2452969-2
