In:
Frontiers in Sustainable Food Systems, Frontiers Media SA, Vol. 6 ( 2022-3-25)
Abstract:
Lesion mimic mutants ( LMM s) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM , making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae ( M . oryzae ). Here, we identified a rice LMM, lm8015-3 , which shows reduced resistance to M . oryzae . We used Quantitative Real-Time PCR (qRT-PCR) to observe the immune system response to M. oryzae –induced lm8015-3 . Lm8015-3 , obtained from an ethyl methane sulfonate (EMS)–induced Zhonghui8015 (ZH8015) library, showed orange-yellow spots starting in the seedling stage and accumulated more H 2 O 2 , resulting in severe degradation of the chloroplast. With map-based cloning, the target gene was located on chromosome 12. Once inoculated with M. oryzae , the expression level of pathogen-related genes of lm8015-3 was downregulated between 48 and 72 h. In addition, more germinating spores appeared in lm8015-3 . Therefore, we conclude that M. oryzae weakening the immune system of lm8015-3 from 48 to 72 h makes lm8015-3 more susceptible to M. oryzae . These results suggested that understanding how LMM s defend against M. oryzae infection will contribute to improving rice breeding.
Type of Medium:
Online Resource
ISSN:
2571-581X
DOI:
10.3389/fsufs.2022.809008
DOI:
10.3389/fsufs.2022.809008.s001
DOI:
10.3389/fsufs.2022.809008.s002
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2928540-9
