In:
Bioengineering, MDPI AG, Vol. 8, No. 8 ( 2021-07-22), p. 103-
Abstract:
Streptomyces clavuligerus (S. clavuligerus) has been widely studied for its ability to produce clavulanic acid (CA), a potent inhibitor of β-lactamase enzymes. In this study, S. clavuligerus cultivated in 2D rocking bioreactor in fed-batch operation produced CA at comparable rates to those observed in stirred tank bioreactors. A reduced model of S. clavuligerus metabolism was constructed by using a bottom-up approach and validated using experimental data. The reduced model was implemented for in silico studies of the metabolic scenarios arisen during the cultivations. Constraint-based analysis confirmed the interrelations between succinate, oxaloacetate, malate, pyruvate, and acetate accumulations at high CA synthesis rates in submerged cultures of S. clavuligerus. Further analysis using shadow prices provided a first view of the metabolites positive and negatively associated with the scenarios of low and high CA production.
Type of Medium:
Online Resource
ISSN:
2306-5354
DOI:
10.3390/bioengineering8080103
Language:
English
Publisher:
MDPI AG
Publication Date:
2021
detail.hit.zdb_id:
2746191-9