In:
Biosensors, MDPI AG, Vol. 13, No. 1 ( 2023-01-13), p. 133-
Kurzfassung:
In general, co-reactants are essential in highly efficient electrochemiluminescence (ECL) systems. Traditional co-reactants are usually toxic, so it is necessary to develop new environmentally friendly co-reactants. In this work, carbon dots (CDs) were assembled with dendritic silica nanospheres (CDs@dSiO2 NPs) to form a co-reactant of Ru(bpy)32+. Subsequently, a sandwich immunosensor for detecting human chorionic gonadotropin (HCG) was constructed based on CDs@dSiO2 NPs as co-reactants, the nanoprobe loaded with the secondary antibody, and Ru(bpy)32+ as a luminophore. In addition, compared to directly as a signal probe, the luminophore Ru (bpy)32+ as a part of the electrolyte solution is simpler in this work. The immunosensor has an extremely low limit of detection of 0.00019 mIU/mL. This work describes the synthesis of low-toxic, efficient, and environmentally friendly CDs, which have become ideal co-reactants of Ru(bpy)32+, and proposes an ECL immunosensor with excellent stability and selectivity, which has great potential in clinical applications.
Materialart:
Online-Ressource
ISSN:
2079-6374
DOI:
10.3390/bios13010133
Sprache:
Englisch
Verlag:
MDPI AG
Publikationsdatum:
2023
ZDB Id:
2662125-3
