In:
Electronics, MDPI AG, Vol. 11, No. 20 ( 2022-10-12), p. 3276-
Abstract:
The effect of a high-voltage discharge in a helicoidal structure on the adsorption properties of an enzyme on mica has been studied with the example of horseradish peroxidase (HRP). The discharge was generated at the expense of a sparkover in a 3 mm gap between two electrodes, to which a 10 kV, 50 Hz AC voltage was applied. The electrodes were connected to a twisted pair, which was wound onto a cone, forming the helicoidal structure. The incubation of the enzyme solution near the top of the helicoidal structure has been found to cause an increase in the degree of aggregation of HRP adsorbed on mica in comparison with the control HRP sample. The results obtained should be taken into account in studies of enzymes using biosensors with helicoidal structures as heating elements, as well as in refining models describing effects of low-frequency alternating current, flowing through helicoidal structures, on proteins and biological objects.
Type of Medium:
Online Resource
ISSN:
2079-9292
DOI:
10.3390/electronics11203276
Language:
English
Publisher:
MDPI AG
Publication Date:
2022
detail.hit.zdb_id:
2662127-7
