In:
Genes, MDPI AG, Vol. 14, No. 2 ( 2023-02-11), p. 467-
Kurzfassung:
Sheep birth and weaning weights indicate their growth and survival. Thus, identifying molecular genetic markers for early body weight is important in sheep breeding. Pleomorphic adenoma gene 1 (PLAG1) is important for regulating birth weight and body length in mammals; however, its relationship with sheep body weight remains unknown. Here, the 3′-untranslated region (3′-UTR) of the Hu sheep PLAG1 gene was cloned, single nucleotide polymorphisms (SNPs) were screened, genotype–early body weight relationships were analyzed, and the possible molecular mechanism was explored. PLAG1 3′-UTR sequences with five forms of base sequences plus poly(A) tails were detected in Hu sheep and the g.8795C 〉 T mutation was identified. Luciferase reporter assay indicated that the g.8795C 〉 T mutation influenced PLAG1 post-transcriptional activity. miRBase prediction showed that the g.8795C 〉 T mutation was located in the miR-139 seed sequence binding region, and miR-139 overexpression significantly decreased both PLAG1-CC and PLAG1-TT activities. Moreover, the luciferase activity of PLAG1-CC was significantly lower than that of the PLAG1-TT, but miR-139 inhibition substantially increased both PLAG1-CC and PLAG1-TT luciferase activities, suggesting that PLAG1 is the target gene of miR-139. Thus, the g.8795C 〉 T mutation upregulates PLAG1 expression by weakening its binding with miR-139, promoting PLAG1 expression, and increasing Hu sheep birth and weaning weights.
Materialart:
Online-Ressource
ISSN:
2073-4425
DOI:
10.3390/genes14020467
Sprache:
Englisch
Verlag:
MDPI AG
Publikationsdatum:
2023
ZDB Id:
2527218-4
