In:
International Journal of Molecular Sciences, MDPI AG, Vol. 25, No. 2 ( 2024-01-19), p. 1249-
Abstract:
Media exposed to atmospheric pressure plasma (APP) produce reactive oxygen and nitrogen species (RONS), with hydrogen peroxide (H2O2), nitrite (NO2−), and nitrate (NO3−) being among the most detected species due to their relatively long lifetime. In this study, a standardized microwave-excited (ME) APP jet (APPJ) source was employed to produce gaseous RONS to treat liquid samples. The source was a commercially available plasma jet, which generated argon plasma utilizing a coaxial transmission line resonator at the operating frequency of 2.45 GHz. An ultraviolet-visible spectrophotometer was used to measure the concentrations of H2O2 and NO3− in plasma-activated media (PAM). Three different types of media (deionized water, Hank’s balanced salt solution, and cell culture solution Dulbecco’s modified eagles medium [DMEM] ) were utilized as liquid samples. Among these media, the plasma-treated DMEM was observed to have the highest levels of H2O2 and NO3−. Subsequently, the feasibility of using argon ME-APPJ-activated DMEM (PAM) as an adjuvant to enhance the therapeutic effects of cisplatin on human bladder cancer cells (T-24) was investigated. Various cancer cell lines, including T-24 cells, treated with PAM were observed in vitro for changes in cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. A viability reduction was detected in the various cancer cells after incubation in PAM. Furthermore, the study’s results revealed that PAM was effective against cisplatin-resistant T-24 cells in vitro. In addition, a possible connection between HER expression and cell viability was sketched.
Type of Medium:
Online Resource
ISSN:
1422-0067
DOI:
10.3390/ijms25021249
Language:
English
Publisher:
MDPI AG
Publication Date:
2024
detail.hit.zdb_id:
2019364-6
SSG:
12
