In:
Marine Drugs, MDPI AG, Vol. 17, No. 11 ( 2019-11-08), p. 633-
Abstract:
In this study, the antioxidant components in co-culture of Chlorella pyrenoidosa and Yarrowia lipolytica (3:1 ratio) were confirmed as trypsin-hydrolyzed peptides (EHPs). The EHPs were composed of 836 different peptides with molecular weights ranging from 639 to 3531 Da and were mainly composed of hydrophobic amino acids (48.1%). These peptides showed remarkable protective effects against oxidative stress in HepG2, which may be attributed to their structures. Furthermore, the mRNA and protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) were significantly lower in the peptide-treated group than in the control group, suggesting that the antioxidant enzyme-coding genes were not activated. The EC50 value of three peptides in the EHPs were in the order of AGYSPIGFVR (0.04 ± 0.002 mg/mL) 〉 VLDELTLAR (0.09 ± 0.001 mg/mL) 〉 LFDPVYLFDQG (0.41 ± 0.03 mg/mL); these results agreed with the prediction of the model (R2 〉 0.9, Q2 〉 0.5). Thus, EHPs show potential as potent new antioxidant agents.
Type of Medium:
Online Resource
ISSN:
1660-3397
Language:
English
Publisher:
MDPI AG
Publication Date:
2019
detail.hit.zdb_id:
2175190-0
SSG:
15,3
