In:
Tropical Medicine and Infectious Disease, MDPI AG, Vol. 7, No. 11 ( 2022-10-22), p. 326-
Abstract:
Many blood-based gene expression biomarkers for monitoring tuberculosis (TB) treatment have been suggested so far, but promising biomarker results for drug-resistant TB treatment response have not been studied. This protocol presents a prospective observational study in Indonesia to profile the human blood transcriptome for predicting the response to drug-resistant TB treatment, focusing on pulmonary TB, and to adapt the specific RNA signature to the qRT-PCR platform. Longitudinal blood samples will be collected from 44 subjects with rifampicin resistant TB, confirmed by Xpert MTB/RIF, and 52 healthy controls. RNA-Seq will be performed to identify changes in the transcriptome following TB treatment. A discriminative RNA signature will be chosen and translated into a score for use in a quantitative PCR-based assay. This study will provide crucial information to guide the discovery and design of a clinically implementable tool to monitor the response of TB treatment.
Type of Medium:
Online Resource
ISSN:
2414-6366
DOI:
10.3390/tropicalmed7110326
Language:
English
Publisher:
MDPI AG
Publication Date:
2022
detail.hit.zdb_id:
2934690-3