In:
Cell Transplantation, SAGE Publications, Vol. 17, No. 12 ( 2008-12), p. 1371-1380
Kurzfassung:
Rheumatoid arthritis is a chronic inflammatory disease. The generation of reactive oxygen species (ROS) within an inflamed joint has been suggested as playing a significant pathogenic role. Extracellular superoxide dismutase (EC-SOD) is a major scavenger enzyme of ROS, which has received growing attention for its therapeutic potential. To investigate the therapeutic effect of EC-SOD in mice with collagen-induced arthritis (CIA), we used mouse embryonic fibroblast (MEF) of transgenic mice that overexpresses EC-SOD on the skin by using hK14 promoter. DBA/1 mice that had been treated with bovine type II collagen were administrated subcutaneous injections of EC-SOD transgenic MEF (each at 1.4 × 10 6 cells) on days 28, 35, and 42 after primary immunization. To test EC-SOD activity, blood samples were collected in each group on day 49. The EC-SOD activity was nearly 1.5-fold higher in the transgenic MEF-treated group than in the non-transgenic MEF-treated group (p 〈 0.05). The severity of arthritis in mice was scored in a double-blind manner, with each paw being assigned a separate clinical score. The severity of arthritis in EC-SOD transgenic MEF-treated mice was significantly suppressed in the arthritic clinical score (p 〈 0.05). To investigate the alteration of cytokine levels, ELISA was used to measure blood samples. Levels of IL-1β and TNF-α were reduced in the transgenic MEF-treated group (p 〈 0.05). Abnormalities of the joints were examined by H & E staining. There were no signs of inflammation except for mild hyperplasia of the synovium in the transgenic MEF-treated group. The proliferation of CII-specific T cells was lower in the transgenic MEF-treated mice than in those in the other groups. The transfer of EC-SOD transgenic MEF has shown a therapeutic effect in CIA mice and this approach may be a safer and more effective form of therapy for rheumatoid arthritis.
Materialart:
Online-Ressource
ISSN:
0963-6897
,
1555-3892
DOI:
10.3727/096368908787648029
Sprache:
Englisch
Verlag:
SAGE Publications
Publikationsdatum:
2008
ZDB Id:
2020466-8
