In:
ZooKeys, Pensoft Publishers, Vol. 1123 ( 2022-10-05), p. 147-171
Kurzfassung:
Barcode libraries are generally assembled with two main objectives in mind: specimen identification and species discovery/delimitation. In this study, the standard COI barcode region was sequenced from 681 specimens belonging to katydids (Tettigoniidae), cave crickets (Rhaphidophoridae), and leaf-rolling crickets (Gryllacrididae) from Zhejiang Province, China. Of these, four COI-5P sequences were excluded from subsequent analyses because they were likely NUMTs (nuclear mitochondrial pseudogenes). The final dataset consisted of 677 barcode sequences representing 90 putative species-level taxa. Automated cluster delineation using the Barcode of Life Data System (BOLD) revealed 118 BINs (Barcodes Index Numbers). Among these 90 species-level taxa, 68 corresponded with morphospecies, while the remaining 22 were identified based on reverse taxonomy using BIN assignment. Thirteen of these morphospecies were represented by a single barcode (so-called singletons), and each of 19 morphospecies were split into more than one BIN. The consensus delimitation scheme yielded 55 Molecular Operational Taxonomic Units (MOTUs). Only four morphospecies ( I max & gt; DNN) failed to be recovered as monophyletic clades (i.e., Elimaea terminalis , Phyllomimus klapperichi , Sinochlora szechwanensis and Xizicus howardi ), so it is speculated that these may be species complexes. Therefore, the diversity of katydids, cave crickets, and leaf-rolling crickets in Zhejiang Province is probably slightly higher than what current taxonomy would suggest.
Materialart:
Online-Ressource
ISSN:
1313-2970
,
1313-2989
DOI:
10.3897/zookeys.1123.86704
DOI:
10.3897/zookeys.1123.86704.figure1
DOI:
10.3897/zookeys.1123.86704.figure2
DOI:
10.3897/zookeys.1123.86704.figure3
DOI:
10.3897/zookeys.1123.86704.figure4
DOI:
10.3897/zookeys.1123.86704.figure5
DOI:
10.3897/zookeys.1123.86704.suppl1
Sprache:
Unbekannt
Verlag:
Pensoft Publishers
Publikationsdatum:
2022
ZDB Id:
2445640-8
SSG:
12
