In:
Applied Mechanics and Materials, Trans Tech Publications, Ltd., Vol. 108 ( 2011-10), p. 189-193
Abstract:
In the course of a search for chemotherapeutic agents inhibiting suspected peridontitis bacteria, extracted and purified substances from lotus leaf were identified by antimicrobial and Anti-inflammation activity tests with use of the broth micro-dilution methods on 96-microwell plate and ELISA assay. The antimicrobial activity of extracts was tested against four microorganisms, namely: Streptococcus mutans , Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Candida albicans. The Anti-inflammation activity of extracts was tested for inhibition of inflammatory cytokines IL-8 and IL-6 in macrophage BF24. And one pure component was subjected to spectroscopic analysis using UV, IR, EMI-MS, EIMS, 1H-NMR, 13C-NMR, 1H-COSY and HMQC. Our data showed that the minimum inhibitory concentrations of the pour component was less than 5mg/ml for all four microorganisms and the minimum concentration with anti-inflammation was 0.2mg/ml for both IL-8 and IL-6. The component was determined to be Lysicamine. Thus, we conclude that Lysicamine extracted from lotus leaves may be a potential antibacterial and Anti-inflammation agent for oral infection .
Type of Medium:
Online Resource
ISSN:
1662-7482
DOI:
10.4028/www.scientific.net/AMM.108
DOI:
10.4028/www.scientific.net/AMM.108.189
Language:
Unknown
Publisher:
Trans Tech Publications, Ltd.
Publication Date:
2011
detail.hit.zdb_id:
2251882-4
