In:
Advanced Materials Research, Trans Tech Publications, Ltd., Vol. 1004-1005 ( 2014-8), p. 837-840
Abstract:
Protein is the major component of muscle tissue, in order to analyze change rule of the grass carp muscle protein during storage, the Plackett–Burman design and response surface methodology were employed to optimize its extraction conditions. The results showed that pH, SDS concentration and liquid-solid ratio were the significant factors influencing response, and the final combination of the optimized parameters was generated: pH, 8.3; liquid-solid ratio, 17:1; SDS concentration, 3.50%;beta mercaptoethanol concentration,0.3%; extraction temperature, 25 °C; extraction time, 1 h; ultrasonic time,15 min. And under these conditions, the protein extraction rate increased to 92.42%.
Type of Medium:
Online Resource
ISSN:
1662-8985
DOI:
10.4028/www.scientific.net/AMR.1004-1005
DOI:
10.4028/www.scientific.net/AMR.1004-1005.837
Language:
Unknown
Publisher:
Trans Tech Publications, Ltd.
Publication Date:
2014
detail.hit.zdb_id:
2265002-7