In:
Advanced Materials Research, Trans Tech Publications, Ltd., Vol. 647 ( 2013-1), p. 476-481
Abstract:
Micro- and nanotopography as well as the surface chemistry of biomaterials affect cell adhesion, proliferation and cell differentiation. Furthermore, the organization and localization of intracellular adhesion components such as the actin cytoskeleton are also altered dependent on the material surface topography. However, the detailed influence of the material micro-structure on cellular mechanisms on the molecular level is still unknown. This study is intended to elucidate such effects using regular pillar structures to characterize the modulation of cell responses like the regulation of voltage-sensitive calcium channels as well as signaling molecules in human osteoblasts. To analyze cell behavior on defined biomaterial surfaces, human osteoblastic MG 63 cells were cultured on geometrically micro textured titanium coated silicon wafers, as opposed to planar titanium references. Samples were fabricated by a photolithographic process using the negative tone resist SU 8 and sputter-coated with 100 nm titanium. Immunofluorescence staining and flow cytometry are used to detect the expression levels and the function of T type calcium channels. Knowledge about the biocomplexity of cell behavior dependent on topographical characteristics is of clinical relevance for the development of implant designs in tissue engineering.
Type of Medium:
Online Resource
ISSN:
1662-8985
DOI:
10.4028/www.scientific.net/AMR.647
DOI:
10.4028/www.scientific.net/AMR.647.476
Language:
Unknown
Publisher:
Trans Tech Publications, Ltd.
Publication Date:
2013
detail.hit.zdb_id:
2265002-7
