In:
Advanced Materials Research, Trans Tech Publications, Ltd., Vol. 726-731 ( 2013-8), p. 396-400
Abstract:
Salt lake environments are economically and ecologically important issues. Like any other ecosystems in the world, salt lake ecosystems are facing various kinds of pollutions. However, little information is available on the biodegradation of organic pollutants by halophilic microorganisms in such environments. In the present work we isolated a strain of phenol-degradating bacteria from a salt lake in Inner Mongolia. Sequence of 16S rRNA suggested that the strain belong to Propionibacterium genus. The strain can grow with phenol as a sole carbon source under 10% (w/v) salt concentration. PCR amplifications with the primers for multicomponent phenol hydroxylase or catechol 1, 2 dioxygenase gene were applied to genome DNA of the strain, suggesting that phenol might be transformed to catechol by multicomponent phenol hydroxylase and further degradated through ortho-ring cleavage pathway. When the primers for catechol 1, 2 dioxygenase were applied to the DNA obtained from the lake water, at least two types of catechol 1, 2 dioxygenase genes sequences were obtained, suggesting the presence of the bacteria with diverse aromatic compound-degradating genes.
Type of Medium:
Online Resource
ISSN:
1662-8985
DOI:
10.4028/www.scientific.net/AMR.726-731
DOI:
10.4028/www.scientific.net/AMR.726-731.396
Language:
Unknown
Publisher:
Trans Tech Publications, Ltd.
Publication Date:
2013
detail.hit.zdb_id:
2265002-7
