In:
The Journal of Immunology, The American Association of Immunologists, Vol. 113, No. 4 ( 1974-10-01), p. 1334-1345
Abstract:
Human lymphotoxin obtained from established lymphoid cell lines (HLT-LCL) was studied in detail and purified using ammonium sulfate fractionation, gel filtration, ion-exchange column chromatography, and polyacrylamide gel electrophoresis. HLT-LCL is stable at 20°C for over 1 year, at 4°C for 3 weeks, and at 37°C for 1 week. HLT-LCL showed a decrease in activity at pH 2.0 and 3.0, but was stable at a pH range of 5.0–11.0 HLT-LCL, which contained fetal calf serum, was purified 50-fold. HLT-LCL obtained from serum-free culture medium yielded greater purity, although complete purification was not achieved. Antiserum to HLT-LCL neutralized the cytotoxic activity of HLT-LCL, HLT induced by PHA (HLT-PHA), and HLT induced by PPD (HLT-PPD). Antiserum to human IgG, λ-type L chain, and Fc fragment partially but significantly inhibited HLT-LCL activity. These data, together with previous reports, indicate that the HLT-LCL molecule is immunologically similar to HLT-PHA and HLT-PPD and suggest that there is immunologic cross-reactivity with IgG globulin.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.113.4.1334
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
1974
detail.hit.zdb_id:
1475085-5